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KMID : 0357319940290010065
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Journal of the Korean Society for Microbiology
1994 Volume.29 No. 1 p.65 ~ p.78
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Rickettsia tsutsugamushi Induced Cellular Injury of Cultured Human Endothelial Cell
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Abstract
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R. tsutsugamushi, the etiologic agent of scrub typhus has been known to cause diffuse vasculitis of small blood vessel and depletion of circulating platelets in peripheral blood of human in infection.
On the basis of the theory that human endothelial cell are main target of R. tsutsugamushi, cellular injury followed by inoculation of R. tsutsugamushi in cultured human endothelial cell was studied by immunofluorescent antibody technique(IF) and
electron microscopy(EM)in order to identify the mode of pathogenesis.
Human endothelial cells were obtained from human umbilical vein after treatment of 0.2% collagenase(13 to 15 min). These cells were cultured to study the pathogenesis of R. tsutsugamushi after identifying von Willebrand factor by indirect IF and
Weibel-Palade body (WPB) by EM. Indirect IF study showed that relatively small number of R. tsutsugamushi in cultured human endothelial cell in 5, 10 and 24 hours after infection. But there were prominent increase in numbers of microorganism in
cultured
cell after 48 hours of infection. R. tustusgamushi at each different stage of pathogenesis could be observed after 48 hours of infection by EM. Ultrastractural changes of endothelial cell in the progress of infection were swelling of
mitochondria,
vacuolization and morphological alteration of WPB. Occassionally, particles implicating R.tsutsugamushi were observed in the nucleus of endothelial cells.
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